Applications
Triacylglycerol Lipids
Acylglycerol Lipids (ESI, APCI, APPI comparison)
Observations
- APPI has 5-decades linearity and is 2-4x more sensitive than APCI and much more sensitive than ESI w/o mobile phase additives for these compounds
- ESI sensitivity enhanced by mobile phase modifiers, but at much reduced linear range
Results recorded on a Waters ZQ. All runs were FIA; 1:1 Iso-octane/IPA; 100 µL/min.
Further Reading
S-S. Cai, L. C. Short, J. A. Syage, M. Potvin, and J. M. Curtis, “APPI-LC/MS analysis of triacylglycerol lipids – effects of mobile phases on sensitivity, J. Chromatogr. A 1173, 88-97 (2007).
S-S. Cai and J. A. Syage, “APPI MS for Analysis of Fatty Acids and Acylglycerol Lipids,” J. Chromatogr. A 1110, 15-26 (2006).
S-S. Cai and J. A. Syage, “Comparison of APPI, APCI, and ESI MS for Analysis of Lipids,” Anal. Chem. 78, 1191-99 (2006).
For additional application notes, please see the Resource Library section of this website.