Applications
Chiral Separations
Acylglycerol Lipids (ESI, APCI, APPI comparison)
Observations
- APPI offers higher peak intensity and lower baseline noise, and therefore higher S/N ratio than APCI
- APPI advantage is dramatically greater for on-column vs. FIA analysis
Results recorded on a Waters ZQ. ChiralPak AD-H 150×2.1 mm, 5 m particle 8:2 hexane/IPA at 0.2 mL/min, isocratic
Further Reading
S-S. Cai, K. A. Hanold, and J. A. Syage, “Comparison of APPI and APCI for Normal Phase LC/MS Chiral Analysis of Pharmaceuticals,” Anal. Chem. 79, 2491-2498 (2007).
For additional application notes, please see the Resource Library section of this website.